Anti-RANKL Anticorps (Biotin) (A282252)

Anti-RANKL Antibody (Biotin) - BSA and Azide free

Rabbit polyclonal antibody to RANKL (Biotin).

This antibody recognises the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.

WB, ELISA, IHC-P

ELISA: 0.15 - 0.30 µg/ml, WB: 0.1 - 0.2 µg/ml

Human

Recombinant human CD254.

Rabbit

Polyclonal

IgG

Biotin

Lyophilized

Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Human CD254 antibody recognizes the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.

CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa B ligand, Receptor activator of nuclear factor kappa-B ligand, sOdf, TNF related activation induced cytokine, TNF-related activation-induced cytokine, TNF11_HUMAN, TNFSF 11, Tnfsf11, TRANCE, Tumor necrosis factor (ligand) superfamily member 11, Tumor necrosis factor ligand superfamily member 11, Tumor necrosis factor ligand superfamily member 11, soluble form

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.