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Anti-Rag A Anticorps (A398)

Ce produit est identique à Abcam (ab128196). Découvrez plus pour moins cher.

Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution. Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilution Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right
$510

Anticorps polyclonal de lapin contre Rag A pour WB.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Rag A Antibody
Description du produit
Rabbit polyclonal antibody to Rag A.
Applications
WB
Recommander des dilutions
WB: 1:1,000-1:2,000
Reactivité
Human, Mouse, Rat, Hamster, Xenopus
Immunogène
Purified full length human Rag A protein (GST tag).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Forme du produit
Liquid
Formulation
Supplied as antiserum with 0.05% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RagA, Ras-related GTP-binding protein A, RRAGA, RRAGA_HUMAN
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody
Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilutionCliquez pour ouvrir le point de vue élargi
Figure 2: SDS-PAGE - Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody
Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on rightCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Rag A Antibody (71-020)
Anti-Rag A Antibody

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