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Anti-Rad52 Anticorps (A101677)

Nom du produit
Anti-Rad52 Antibody
Description du produit
Rabbit polyclonal antibody to Rad52.
Applications
WB, IP, IF, ELISA
Recommander des dilutions
WB: 1:1,000-1:3,000, IP: 1:50-1:1,000, IF: 1:1,000
Reactivité
Human, Mouse, Rat, Hamster
Immunogène
Recombinant full length human Rad52 protein.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in 20mM Phosphate Buffered Saline, pH 7.5, with 0.15M Sodium Chloride and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
DNA repair protein RAD52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog, RAD52 homolog (S. cerevisiae), RAD52_HUMAN, Recombination protein RAD52, Rhabdomyosarcoma antigen MU RMS 40.23
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot of Rad52 in mammalian cells. M: Marker proteins. Lane 1: Recombinant human Rad52 protein (0.2ng). Lane 2: HeLa cell extrat (17µg). Lane 3: MCF7 cell extract (27µg). Lane 4: NIH3T3 cell extract (20µg). Lane 5: CHO cell extract (21µg). The samples were separated by electrophoresis on 10% SDS-PAGE and blotted onto PVDF membrane. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Molecular mass of human Rad52 protein is 47 kDa.Cliquez pour ouvrir le point de vue élargi
Figure 1: SDS-PAGE - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
Western blot of Rad52 in Rat cells. Lane 1: Human MCF-7 cell lysate (16µg). Lane 2: Rat PC-12 cell (40µg). Proteins in the lysates were separated on SDS-PAGE (12.5%) and electro-blotted to PVDF membrane overnight. The blot was blocked with 5% skim milk. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution.Cliquez pour ouvrir le point de vue élargi
Figure 2: SDS-PAGE - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
Immunoprecipitation of Rad52 from human cell lysate. 1. Add 10µg of Anti-Rad52 Antibody and 50µg of Dynabeads protein A. 2. Incubate for 10 min at room temperature, wash and resuspend in 100µl of the same buffer. 3. Add 100µl (300µg in PBS) lysate of human MCF7 cells. 4. Incubate for and collect the immunocomplex with Dynabeeds magnetic apparatus. 5. Elute the immunocomplex and analyze the presence of Rad52 in the supernatant (Lane 2) and the eluate (Lane 1) by western blotting with Anti-Rad52 Antibody. 6. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 3 is western blot of MCF’ lysate (16µg).Cliquez pour ouvrir le point de vue élargi
Figure 3: Immunoprecipitation - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
HU-induced focus formation of Rad52. U2OS 40-2-6 cells were untreated or treated with 2mM hydroxyurea (HU) for 24 h. The cells were pre-extracted with 0.1% TritonX-100 in PBS, fixed with 3.7% formaldehyde in PBS, and immunostained with Anti-Rad52 Antibody (green) at 1:50 dilution, followed by DAPI staining (blue). Goat Anti-Rabbit IgG Antibody (CF488A) was used as a secondary at 1:1,000 dilution. TritonX-100 treatment extracts chromatin-free Rad52 from nuclei. Scale bars, 20 µm.Cliquez pour ouvrir le point de vue élargi
Figure 4: Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody

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