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Anti-Rad18 Anticorps (A354)

Identification of mouse Rad18 protein in ES cells by western blotting with Anti-Rad18 Antibody. WT: Lysate of wild-type mouse ES cells RAD18-/- ; Lysate of Rad18 double knock-out mouse ES cells. Protein levels of alpha Tubulin in the lysates are shown as a control. Three bands are absent in RAD18 knock-out cells. Identification of mouse Rad18 protein in NIH3T3 cells by western blotting with Anti-Rad18 Antibody. Cell extract, 23µg, was used. Anti-Rad18 Antibody was used at 1:2,000 dilution. Two extra bands (75 + 90 kDa) may represent modified products. Immunofluorescence staining of Rad18 protein with Anti-Rad18 Antibody. Wild-type (+/+) and RAD18-/- ES cells (-/-). Samples were prefixed 3.7% formaldehyde and fixed with 80% methanol. Anti-Rad18 Antibody was used at 1:300 dilution. Anti-Rabbit IgG (FITC) was used as a secondary antibody. Detection of Rad18 in mouse testis. Section of paraformaldehyde fixed mouse was stained with Anti-Rad18 Antibody. Donkey Anti-Rabbit IgG Antibody (HRP) was used as a secondary antibody. Signals were enhanced with TSA plus biotin system and detected by using DAB substrate. Arrows indicate undifferentiated spermatogonia.
Anticorps Validé par Knockout
$535

Anticorps polyclonal de lapin contre Rad18 pour WB, IP, IF et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Rad18 Antibody
Description du produit
Rabbit polyclonal antibody to Rad18.
Applications
WB, IP, IF, IHC
Recommander des dilutions
WB: 1:1,000, IP: 1:200-1:500, IHC: 1:100-1:300
Reactivité
Mouse
Immunogène
Synthetic peptide containing 100 carboxyl terminal amino acids of mouse Rad18 protein (GST tag).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
2810024C04Rik, DNA repair protein rad18, E3 ubiquitin-protein ligase RAD18, EC 6.3.2.-, hHR 18, hHR18, hRAD 18, hRAD18, MGC156682, Postreplication repair E3 ubiquitin-protein ligase RAD18, Postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD 18, RAD18 homolog, RAD18 homolog (S. cerevisiae), RAD18 S. cerevisiae homolog, RAD18 S. cerevisiae homolog of, RAD18 transcript variant, Rad18sc, RAD18_HUMAN, Radiation sensitivity protein 18, RING finger protein 73, RNF 73, RNF73, Structural maintenance of chromosomes protein 6, YCR066W, YCR66W
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Identification of mouse Rad18 protein in ES cells by western blotting with Anti-Rad18 Antibody. WT: Lysate of wild-type mouse ES cells RAD18-/- ; Lysate of Rad18 double knock-out mouse ES cells. Protein levels of alpha Tubulin in the lysates are shown as a control. Three bands are absent in RAD18 knock-out cells.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Identification of mouse Rad18 protein in NIH3T3 cells by western blotting with Anti-Rad18 Antibody. Cell extract, 23µg, was used. Anti-Rad18 Antibody was used at 1:2,000 dilution. Two extra bands (75 + 90 kDa) may represent modified products.Cliquez pour ouvrir le point de vue élargi
Figure 2: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Immunofluorescence staining of Rad18 protein with Anti-Rad18 Antibody. Wild-type (+/+) and RAD18-/- ES cells (-/-). Samples were prefixed 3.7% formaldehyde and fixed with 80% methanol. Anti-Rad18 Antibody was used at 1:300 dilution. Anti-Rabbit IgG (FITC) was used as a secondary antibody.Cliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Detection of Rad18 in mouse testis. Section of paraformaldehyde fixed mouse was stained with Anti-Rad18 Antibody. Donkey Anti-Rabbit IgG Antibody (HRP) was used as a secondary antibody. Signals were enhanced with TSA plus biotin system and detected by using DAB substrate. Arrows indicate undifferentiated spermatogonia.Cliquez pour ouvrir le point de vue élargi
Figure 4: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody

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