Figure 1: Western Blot - Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276)
Western blot of non-reduced (-) and reduced (+) rabbit IgG (20ng/lane), using 0.2µg/ml of Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin). This antibody reacts to non-reduced rabbit IgG (~150 kDa).
ELISA of IgGs from different species, shows Anti-Rabbit IgG F(ab) Antibody [RMG01] reacts to the Fab region of rabbit IgG. There is no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Rabbit IgG F(ab) Antibody [RMG01] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Anti-Rabbit IgG F(ab) Antibody [RMG01] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.