Anti-QKI Anticorps [S147-6] (A304994)

Name: Anti-QKI Antibody [S147-6]
Brand: Antibodies.com
SKU: A304994
Price: 500.0 USD
Availability: InStock

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$500

Anticorps monoclonal [S147-6] de souris contre QKI pour WB, IHC et ICC/IF.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-QKI Antibody [S147-6]

Description du produit

Mouse monoclonal [S147-6] antibody to QKI.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b..

Hôte

Mouse

Clonalité

Monoclonal

Clone

S147-6

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~36-38 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZp586I0923, HKQ, Homolog of mouse quaking QKI KH domain RNA binding protein, Hqk, HQK1, HqkI, OTTHUMP00000017581, OTTHUMP00000017582, OTTHUMP00000017583, Protein quaking, QK, QK1, QK3, QKI1, QKI_HUMAN, Quaking homolog, Quaking homolog KH domain RNA binding, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-QKI Antibody [S147-6] (A304994)

Western blot analysis of rat brain membrane showing detection of 36-38 kDa QKI (pan) protein using Anti-QKI Antibody [S147-6] (A304994) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 36-38 kDa.

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Figure 2: Immunofluorescence - Anti-QKI Antibody [S147-6] (A304994)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-QKI Antibody [S147-6] (A304994), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) QKI (pan) Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-QKI Antibody [S147-6] (A304994)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-QKI Antibody [S147-6] (A304994), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI (pan) Antibody (D) Composite.