Anti-QKI Anticorps [PCRP-QKI-2F10] (A278440)

Name: Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free
Brand: Antibodies.com
SKU: A278440
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [PCRP-QKI-2F10] de souris contre QKI pour ELISA, Flow Cytometry, IP, IF et WB.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Monospécifique

Spécifications

Nom du produit

Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free

Description du produit

Mouse monoclonal [PCRP-QKI-2F10] antibody to QKI.

Spécificité

QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Applications

ELISA, Flow Cytometry, IP, IF, WB

Recommander des dilutions

Flow Cytometry: 1-2 µg/million cells, IP: 1-2µg / 100-500µg proteins, IF: 1-2 µg/ml, WB: 1-2 µg/ml

Reactivité

Human

Immunogène

Recombinant full-length human QKI protein.

Hôte

Mouse

Clonalité

Monoclonal

Clone

PCRP-QKI-2F10

Isotype

IgG2c

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Masse moléculaire

37.6 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-QKI Antibody [PCRP-QKI-2F10] (A277852).

Synonymes

DKFZp586I0923, HKQ, Homolog of mouse quaking QKI KH domain RNA binding protein, Hqk, HQK1, HqkI, OTTHUMP00000017581, OTTHUMP00000017582, OTTHUMP00000017583, Protein quaking, QK, QK1, QK3, QKI1, QKI_HUMAN, Quaking homolog, Quaking homolog KH domain RNA binding, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (9)

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Figure 1: SDS-PAGE - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

SDS-PAGE analysis of Anti-QKI Antibody [PCRP-QKI-2F10] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 2: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 3: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed K562 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 4: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed U87 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 5: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed MCF-7 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 6: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of PFA-fixed U87 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 7: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of K562 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 8: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of PFA-fixed MCF-7 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 9: Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-QKI Antibody [PCRP-QKI-2F10]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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