Anti-PUMA Antibody (A88681)

Name: Anti-PUMA Antibody
Brand: Antibodies.com
SKU: A88681
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to PUMA for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PUMA Antibody

Description

Rabbit polyclonal antibody to PUMA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human PUMA (NP_001120713.1).

Sequence

PGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BBC3, Bcl-2-binding component 3, isoforms 1/2, JFY-1, p53 up-regulated modulator of apoptosis

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PUMA Antibody (A88681)

Western blot analysis of extracts of various cell lines, using Anti-PUMA Antibody (A88681) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-PUMA Antibody (A88681)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PUMA Antibody (A88681) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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