Anti-PRPF31 Anticorps (A84273)

Name: Anti-PRPF31 Antibody
Brand: Antibodies.com
SKU: A84273
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab58588). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre PRPF31 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-PRPF31 Antibody

Description du produit

Goat polyclonal antibody to PRPF31.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human PRPF31.

Séquence

KELGNSLDKCKNNEN

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

60 kDa

MW prédit

55.4kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

DKFZp566J153, hPrp 31, hPrp31, NY BR 99, Pre mRNA processing factor 31, Pre mRNA processing factor 31 homolog, Pre mRNA processing factor 31 homolog (yeast), Pre-mRNA-processing factor 31, Precursor mRNA-processing factor 31, S. cerevisiae, homolog of, Protein 61K, PRP 31, PRP31, PRP31 pre mRNA processing factor 31 homolog, PRP31 pre mRNA processing factor 31 homolog (yeast), PRP31_HUMAN, PRPF 31, RP 11, RP11, Serologically defined breast cancer antigen NY BR 99, Serologically defined breast cancer antigen NY-BR-99, SNRNP61, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6 snRNP 61 kDa protein

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in NIH3T3 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in HEK293 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Some cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.