Anti-PRAS40 Anticorps (A11941)

Name: Anti-PRAS40 Antibody
Brand: Antibodies.com
SKU: A11941
Price: 335.0 USD
Availability: InStock

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$335

Anticorps polyclonal de lapin contre PRAS40 pour WB, IHC et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-PRAS40 Antibody

Description du produit

Rabbit polyclonal antibody to PRAS40.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (NP_001092103.1).

Séquence

STDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

50 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, AKT1 S1, AKT1 substrate 1, AKT1 substrate 1 (proline rich), AKT1S 1, AKT1S1, AKTS1_HUMAN, Lobe, MGC2865, PRAS, PRAS 40, Proline rich akt substrate, Proline rich Akt substrate 40 kDa, Proline-rich AKT1 substrate 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-PRAS40 Antibody (A11941)

Western blot analysis of extracts of various cell lines, using Anti-PRAS40 Antibody (A11941) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-PRAS40 Antibody (A11941)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PRAS40 Antibody (A11941)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-PRAS40 Antibody (A11941)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PRAS40 Antibody (A11941) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).