Porcin IP10 Kit Elisa (A327094)

Porcine IP10 ELISA Kit

Porcine IP10 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of porcine IP10 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Porcine IP10 ELISA Kit (A327094) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine IP10 in serum, plasma, and other biological fluids. An antibody specific for IP10 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IP10 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IP10 Antibody, which binds the captured IP10 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IP10 captured in each well. The concentration of IP10 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

18.75 pg/ml

31.25-2000 pg/ml

Porcine

No significant cross-reactivity or interference between porcine IP10 and analogues was observed.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

10 kDa interferon gamma induced protein, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10, C7, Chemokine (C X C motif) ligand 10, Chemokine CXC motif ligand 10, Crg 2, CRG2, CXCL10, CXCL10(1-73), CXL10_HUMAN, Gamma IP10, Gamma-IP10, gIP 10, GIP10, IFI10, INP 10, INP10, Interferon activated gene 10 , Interferon gamma induced cell line, Interferon gamma induced factor MOB1, mouse, homolog of, Interferon gamma induced protein 10, Interferon inducible cytokine IP 10, Interferon inducible cytokine IP10, IP 10, IP-10, Mob 1, MOB1, Protein 10 from interferon (gamma) induced cell line, SCYB10, Small inducible cytokine B10, Small inducible cytokine B10 precursor, Small inducible cytokine subfamily B (Cys X Cys) member 10, Small inducible cytokine subfamily B CXC member 10 , Small inducible cytokine subfamily B, member 10, Small-inducible cytokine B10