Anti-PKC alpha Anticorps [ARC0197] (A13227)

Name: Anti-PKC alpha Antibody [ARC0197]
Brand: Antibodies.com
SKU: A13227
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC0197] de lapin contre PKC alpha pour WB, IHC et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-PKC alpha Antibody [ARC0197]

Description du produit

Rabbit monoclonal [ARC0197] antibody to PKC alpha.

Applications

WB, IHC, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 573-672 of human PKC alpha (P17252).

Séquence

MTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0197

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

76 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AAG6, Aging associated gene 6, aPKC, KPCA_HUMAN, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)

Western blot analysis of extracts of various cell lines, using Anti-PKC alpha Antibody [ARC0197] (A13227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-PKC alpha Antibody [ARC0197] (A13227)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-PKC alpha Antibody [ARC0197] (A13227). This Western blot was performed on the immunoprecipitate using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:1000.