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PIP5K Cell Based Kit Elisa (A103447)

Nom du produit
PIP5K Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This PIP5K Cell Based ELISA Kit allows for the detection of PIP5K and the effects that certain stimulation conditions have on PIP5K expression in different cell lines. Qualitative determination of PIP5K concentration is achieved by an indirect ELISA format. In essence, the PIP5K is captured by Anti-PIP5K Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PIP5K Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
1 phosphatidylinositol 3 phosphate 5 kinase, 1 phosphatidylinositol 4 phosphate 5 kinase, 1-phosphatidylinositol 3-phosphate 5-kinase, CFD, epididymis luminal protein 37, FAB1, FAB1, S. cerevisiae, homolog of, FYV1_HUMAN, FYVE finger containing phosphoinositide kinase, FYVE finger-containing phosphoinositide kinase, KIAA0981, MGC40423, p235, Phosphatidylinositol 3 phosphate 5 kinase type III, Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III, Phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase type III, Phosphatidylinositol 4 phosphate 5 kinase type III, Phosphoinositide kinase, FYVE finger containing, PIKfyve, PIP5K3, PIPkin III, PIPkin-III, PtdIns(4)P 5 kinase, Type III PIP kinase, ZFYVE29, Zinc finger, FYVE domain containing 29
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - PIP5K Cell Based ELISA Kit (CB5559) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - PIP5K Cell Based ELISA Kit (CB5559)
Western blot analysis of extracts from HepG2 cells, using Anti-PIP5K Antibody.
Protocol Diagram - PIP5K Cell Based ELISA Kit (CB5559) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: PIP5K Cell Based ELISA Kit (CB5559)
A diagram showing the protocol for PIP5K Cell Based ELISA Kit.
Protocol Summary - PIP5K Cell Based ELISA Kit (CB5559) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: PIP5K Cell Based ELISA Kit (CB5559)
A summary of the protocol for PIP5K Cell Based ELISA Kit.
Western Blot - Control Antibody - PIP5K Cell Based ELISA Kit (CB5559) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - PIP5K Cell Based ELISA Kit (CB5559)
The mouse monoclonal antibody to GAPDH is used as a positive control inPIP5K Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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