Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499)

Name: Anti-PI3 Kinase p110 beta Antibody [ARC54484]
Brand: Antibodies.com
SKU: A306499
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC54484] antibody to PI3 Kinase p110 beta for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PI3 Kinase p110 beta Antibody [ARC54484]

Description

Rabbit monoclonal [ARC54484] antibody to PI3 Kinase p110 beta.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 410-600 of human PI3 Kinase p110 beta (NP_006210.1).

Sequence

KVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWTLRQDCREIFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC54484

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499)

Western blot analysis of various lysates, using Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499)

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Immunohistochemistry - Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-PI3 Kinase p110 beta Antibody [ARC54484] (A306499) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.