Recombinant Anti-Phosphothreonine Anticorps [RM111] (A121324)

Immunocytochemistry of serum-starved A431 cells non-treated or treated with EGF, using Anti-Phosphothreonine Antibody [RM111] at 1:500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

Western blot of serum-starved A431 cells non-treated or treated with EGF, using Anti-Phosphothreonine Antibody [RM111] at a 1:5,000 dilution. The blot was exposed to the film at different time points.

Immunoprecipitation of EGF-treated A431 cells by Anti-Phosphothreonine Antibody [RM111] at 1:1,000 dilution, was blotted with an Anti-EGFR Antibody. (1) Whole lysate control. (2) IP by rabbit IgG control. (3) IP by Anti-Phosphothreonine Antibody [RM111].

Luminex analysis of Anti-Phosphothreonine Antibody [RM111]’s reaction against phosphorylated or non-phosphorylated Serine, Threonine, and Tyrosine. Anti-Phosphothreonine Antibody [RM111] specifically reacts to phosphorylated Tyrosine.