Recombinant rabbit monoclonal [PRF1/7077R] antibody to Perforin.
Spécificité
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Applications
IHC-P
Recommander des dilutions
IHC-P: 1-2 µg/ml
Reactivité
Human
Immunogène
Recombinant fragment, around amino acids 413-552, of human Perforin 1. The exact sequence is proprietary.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
PRF1/7077R
Isotype
IgG
Chaînes légères
kappa
Conjuguer
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Masse moléculaire
75 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue using Anti-Perforin Antibody [PRF1/7077R]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
SDS-PAGE analysis of Anti-Perforin Antibody [PRF1/7077R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Produits alternatifs à Anti-Perforin Anticorps [PRF1/7077R] (A278049)