Anti-PDHA1 (phospho Ser293) Anticorps [ARC53489] (A306031)

Name: Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]
Brand: Antibodies.com
SKU: A306031
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC53489] de lapin contre PDHA1 (phospho Ser293) pour WB, ICC/IF et IP.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Spécifications

Nom du produit

Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]

Description du produit

Rabbit monoclonal [ARC53489] antibody to PDHA1 (phospho Ser293).

Applications

WB, ICC/IF, IP

Recommander des dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).

Séquence

GHSMS

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC53489

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

43 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

mitochondrial, ODPA_HUMAN, PDH, PDHA, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex E1 alpha polypeptide 1, Pyruvate dehydrogenase complex, E1 alpha polypeptide 1, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, Pyruvate Dehydrogenase E1-alpha subunit, Pyruvate dehydrogenase, alpha-1, somatic form

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of various cell lines, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:1,000 dilution. Hela cells and NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:115000 dilution. 293T cells were treated by Î»-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:20,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Immunofluorescence analysis of NIH/3T3 using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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A89894

Anti-PDHA1 (phospho Ser293) Anticorps [ARC53489] (A306031)

Anticorps monoclonal [ARC53489] de lapin contre PDHA1 (phospho Ser293) pour WB, ICC/IF et IP.

Informations sur la Livraison

Livraison

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Produits alternatifs à Anti-PDHA1 (phospho Ser293) Anticorps [ARC53489] (A306031)