This antibody recognises the carboxy-terminal region of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility.Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.

Applications

WB, IHC-P, ELISA

Recommander des dilutions

ELISA: 1:10,000 - 1:50,000, IHC-P: 1:100 - 1:300, WB: 1:1,000 - 1:10,000

Reactivité

Human, Rat, Mouse, Xenopus

Immunogène

Synthetic peptide corresponding to a region near the C-terminus of human Pdcd4.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Concentration

1 mg/ml

Masse moléculaire

Approximately 52 kDa.

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Stockage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Notes générales

Rabbit anti Human PDCD4 antibody recognizes the carboxy-terminal region of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.

Synonymes

Death up-regulated gene protein, Dug, H731, Ma3, MGC33046, MGC33047, Neoplastic transformation inhibitor, Neoplastic transformation inhibitor protein, Nuclear antigen H731, Nuclear antigen H731 like, Nuclear antigen H731 like protein, Nuclear antigen H731-like, PDCD 4, PDCD4_HUMAN, Programmed cell death 4, programmed cell death 4 (neoplastic transformation inhibitor), Programmed cell death protein 4, Protein 197/15a, Protein MA-3, Tis, Topoisomerase-inhibitor suppressed protein

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.