Anti-PD-L1 Anticorps (A82538)

Garantie de Correspondance des Prix

$480

Anticorps polyclonal de chèvre contre PD-L1 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Alternative Formats and ConjugatesAlternative Formats

Biotin

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-PD-L1 Antibody

Description du produit

Goat polyclonal antibody to PD-L1.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.01-0.1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the C terminal region of human PD-L1.

Séquence

CKKQSDTHLEET

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

50 kDa

MW prédit

33.3kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

Figure 1: Western Blot - Anti-PD-L1 Antibody (A82538)

PD-L1 expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PD-L1 Antibody (A82538) at 0.01µg/ml and detected by chemiluminescence.

Figure 2: Western Blot - Anti-PD-L1 Antibody (A82538)

PD-L1 expression in U2OS cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PD-L1 Antibody (A82538) at 0.1µg/ml and detected by chemiluminescence.

Figure 3: Immunofluorescence - Anti-PD-L1 Antibody (A82538)

PD-L1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PD-L1 Antibody (A82538) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Figure 4: Immunofluorescence - Anti-PD-L1 Antibody (A82538)

PD-L1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PD-L1 Antibody (A82538) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Voir plus de photos

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