Anti-PBX1 Anticorps [PCRP-PBX1-3C8] (A277732)

Name: Anti-PBX1 Antibody [PCRP-PBX1-3C8]
Brand: Antibodies.com
SKU: A277732
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [PCRP-PBX1-3C8] de souris contre PBX1 pour ELISA, IP, Flow Cytometry et IF.

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Carrier Free and Preservative Free

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Mots clés :

Anticorps Monospécifique

Spécifications

Nom du produit

Anti-PBX1 Antibody [PCRP-PBX1-3C8]

Description du produit

Mouse monoclonal [PCRP-PBX1-3C8] antibody to PBX1.

Spécificité

Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92% and 94% respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Applications

ELISA, IP, Flow Cytometry, IF

Recommander des dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivité

Human

Immunogène

Recombinant full-length human PBX1 protein.

Hôte

Mouse

Clonalité

Monoclonal

Clone

PCRP-PBX1-3C8

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

200 µg/ml

Masse moléculaire

86.1 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320).

Synonymes

DKFZp686B09108, Homeo box protein PBX1, Homeo box protein PRL, Homeobox protein PBX 1, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, PBX 1, PBX1_HUMAN, Pre B cell leukemia homeobox 1, Pre B cell leukemia transcription factor 1, Pre-B-cell leukemia transcription factor 1, PRL

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: SDS-PAGE - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

SDS-PAGE analysis of Anti-PBX1 Antibody [PCRP-PBX1-3C8] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 2: Flow Cytometry - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 3: Immunofluorescence - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

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Figure 4: Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-PBX1 Antibody [PCRP-PBX1-3C8]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.