Anti-PARP1 Anticorps [ARC0075] (A306436)

Name: Anti-PARP1 Antibody [ARC0075]
Brand: Antibodies.com
SKU: A306436
Price: 470.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$470

Anticorps monoclonal [ARC0075] de lapin contre PARP1 pour WB et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-PARP1 Antibody [ARC0075]

Description du produit

Rabbit monoclonal [ARC0075] antibody to PARP1.

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PARP1 (P09874).

Séquence

KLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTD

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0075

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

89 kDa / 113 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

ADP ribosyltransferase, ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase), ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD(+), ADPRT, ADPRT 1, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, NAD(+) ADP-ribosyltransferase 1, pADPRT 1, pADPRT1, PARP, PARP 1, PARP-1, PARP1_HUMAN, Poly (ADP ribose) polymerase 1, poly (ADP ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, Poly(ADP ribose) polymerase, poly(ADP ribose) synthetase, poly(ADP ribosyl)transferase, Poly[ADP ribose] synthetase 1, Poly[ADP-ribose] synthase 1, PPOL

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-PARP1 Antibody [ARC0075] (A306436)

Western blot analysis of extracts of Jurkat cells, using Anti-PARP1 Antibody [ARC0075] (A306436) at 1:1,000 dilution. Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-PARP1 Antibody [ARC0075] (A306436)

Western blot analysis of extracts of various cell lines, using Anti-PARP1 Antibody [ARC0075] (A306436) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunohistochemistry - Anti-PARP1 Antibody [ARC0075] (A306436)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-PARP1 Antibody [ARC0075] (A306436) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-PARP1 Antibody [ARC0075] (A306436)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-PARP1 Antibody [ARC0075] (A306436) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.