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Anti-PABPN1 Anticorps [ARC0730] (A306775)

Nom du produit
Anti-PABPN1 Antibody [ARC0730]
Description du produit
Rabbit monoclonal [ARC0730] antibody to PABPN1.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42).
Séquence
MAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGSQEEEE
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0730
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
50 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Nuclear poly(A)-binding protein 1, OPMD, PAB2, PABII, PABP 2, pABP-2, PABP2, PABP2_HUMAN, PABPII, poly(A) binding protein nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, PolyA binding protein II, Polyadenylate-binding nuclear protein 1, Polyadenylate-binding protein 2
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-PABPN1 Antibody [ARC0730] (A306775) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-PABPN1 Antibody [ARC0730] (A306775)
Western blot analysis of extracts of various cell lines, using Anti-polyclonal antibodyPN1 Antibody [ARC0730] (A306775) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-polyclonal antibodyPN1 Antibody [ARC0730] (A306775) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775)
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-polyclonal antibodyPN1 Antibody [ARC0730] (A306775) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-PABPN1 Antibody [ARC0730] (A306775)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-polyclonal antibodyPN1 Antibody [ARC0730] (A306775) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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