Recombinant Anti-p53 Anticorps [TP53/2092R] (A253372)

Name: Anti-p53 Antibody [TP53/2092R] - BSA and Azide free
Brand: Antibodies.com
SKU: A253372
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [TP53/2092R] de lapin contre p53 pour WB et IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Monospécifique Anticorps Recombinant ER Marker Mitochondrial Marker

Spécifications

Nom du produit

Anti-p53 Antibody [TP53/2092R] - BSA and Azide free

Description du produit

Recombinant rabbit monoclonal [TP53/2092R] antibody to p53.

Spécificité

This antibody reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Applications

WB, IHC-P

Recommander des dilutions

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reactivité

Human, Monkey, Canine, Hamster, Chicken

Réactivité croisée

This antibody does not cross react with Mouse or Rat.

Immunogène

Recombinant full-length human TP53 protein.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

TP53/2092R

Isotype

IgG

Conjuguer

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Masse moléculaire

53 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-p53 Antibody [TP53/2092R] (A250192).

Synonymes

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunohistochemistry - Anti-p53 Antibody [TP53/2092R] - BSA and Azide free (A253372)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma using Anti-p53 Antibody [TP53/2092R].

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Figure 2: Western Blot - Anti-p53 Antibody [TP53/2092R] - BSA and Azide free (A253372)

Western blot analysis of HeLa cell lysate using Anti-p53 Antibody [TP53/2092R].

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Figure 3: SDS-PAGE - Anti-p53 Antibody [TP53/2092R] - BSA and Azide free (A253372)

SDS-PAGE analysis of Anti-p53 Antibody [TP53/2092R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 4: Anti-p53 Antibody [TP53/2092R] - BSA and Azide free (A253372)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-p53 Antibody [TP53/2092R]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Produits alternatifs à Anti-p53 Anticorps [TP53/2092R] (A253372)

(4)

A98895

Recombinant Anti-p53 Anticorps [TP53/2092R] (A253372)

Anticorps monoclonal [TP53/2092R] de lapin contre p53 pour WB et IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Informations sur la Livraison

Livraison

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Produits alternatifs à Anti-p53 Anticorps [TP53/2092R] (A253372)