Anti-p53 Antibody [BP53-12] (A86031)

Name: Anti-p53 Antibody [BP53-12]
Brand: Antibodies.com
SKU: A86031
Price: 200.0 USD
Availability: InStock

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$200

Mouse monoclonal [BP53-12] antibody to p53 for Flow Cytometry, IP, WB, IHC-P, ICC and ELISA.

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Alternative Formats and ConjugatesAlternative Formats

FITC

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 Antibody [BP53-12]

Description

Mouse monoclonal [BP53-12] antibody to p53.

Specificity

This antibody recognizes a defined epitope, amino acids 16-25, on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.

Applications

Flow Cytometry, IP, WB, IHC-P, ICC, ELISA

Dilutions

WB: 1-2 µg/ml, overnight at 4°C; Positive Control: RAMOS human lymphoma cell line, Non-reducing conditions. SDS-PAGE (12% separating gel), IHC: 5-10 µg/ml, Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reactivity

Human, Non-Human Primates

Immunogen

Bacterially expressed full length wild-type p53 protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

BP53-12

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Purified by differential precipitation and solid-phase chromatography.

Concentration

1 mg/ml

Predicted MW

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Immunocytochemistry - Anti-p53 Antibody [BP53-12] (A86031)

Confocal microscopy of human HeLa cells using Anti-p53 Antibody (A86031). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with Anti-p53 Antibody (A86031). Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic.

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