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P50 Dynamitin Cell Based Kit Elisa (A102843)

Nom du produit
p50 Dynamitin Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This p50 Dynamitin Cell Based ELISA Kit allows for the detection of p50 Dynamitin and the effects that certain stimulation conditions have on p50 Dynamitin expression in different cell lines. Qualitative determination of p50 Dynamitin concentration is achieved by an indirect ELISA format. In essence, the p50 Dynamitin is captured by Anti-p50 Dynamitin Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this p50 Dynamitin Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
50 kD dynein associated polypeptide, 50 kDa dynein associated polypeptide, 50 kDa dynein-associated polypeptide, DCTN-50, DCTN2, DCTN2_HUMAN, DCTN50, dynactin 2, dynactin 2 (p50), dynactin complex 50 kD subunit, Dynactin complex 50 kDa subunit, Dynactin subunit 2, Dynamitin, RBP50
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - p50 Dynamitin Cell Based ELISA Kit (CB5517) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - p50 Dynamitin Cell Based ELISA Kit (CB5517)
Western blot analysis of extracts from A549 cells, using Anti-p50 Dynamitin Antibody.
Protocol Diagram - p50 Dynamitin Cell Based ELISA Kit (CB5517) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: p50 Dynamitin Cell Based ELISA Kit (CB5517)
A diagram showing the protocol for p50 Dynamitin Cell Based ELISA Kit.
Protocol Summary - p50 Dynamitin Cell Based ELISA Kit (CB5517) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: p50 Dynamitin Cell Based ELISA Kit (CB5517)
A summary of the protocol for p50 Dynamitin Cell Based ELISA Kit.
Western Blot - Control Antibody - p50 Dynamitin Cell Based ELISA Kit (CB5517) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - p50 Dynamitin Cell Based ELISA Kit (CB5517)
The mouse monoclonal antibody to GAPDH is used as a positive control inp50 Dynamitin Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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