Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
DKFZp686A11164, KIAA1451, MGC126578, MGC133203, MST120, MSTP120, ORP 8, OSBP related protein 8, OSBP10, OSBPL8, Oxysterol binding protein like 8, Oxysterol binding protein like protein 8, Oxysterol binding protein related protein 8
Figure 1: Western Blot - Anti-ORP8 Antibody (A307228)
Western blot analysis of extracts of various cell lines, using Anti-ORP8 Antibody (A307228) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-ORP8 Antibody (A307228) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.