Western blot analysis of extracts of various cell lines, using Anti-Optineurin Antibody (A13697) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Optineurin Antibody (A13697) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 25µg extracts of mouse heart cells using 3µg of Anti-Optineurin Antibody (A13697). This Western blot was performed on the immunoprecipitate using Anti-Optineurin Antibody (A13697) at a dilution of 1:1000.
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