This kit relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected ( Ex/Em = 555nm/565 nm).
Type d'échantillon
Cells and tissues.
Composants
TdT Enzyme, Equilibration Buffer (5X), Label Mix Orange, DAPI (500X), BSA Working Solution, TritonX-100 (100%), DNase I (5 U/µl)
Maxima d'absorption
555 nm
Maxima d'émission
565 nm
Stockage
Shipped at 4°C. Store at -20°C. Once opened, refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
