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Anti-NUP98 Anticorps [2H10] (A378)

Ce produit est identique à Abcam (ab50610). Découvrez plus pour moins cher.

Nom du produit
Anti-NUP98 Antibody [2H10]
Description du produit
Rat monoclonal (2H10) antibody to NUP98.
Applications
WB, DB, ICC/IF, ELISA, IP
Reactivité
Human, Mouse, Rat, Schizosaccharomyces pombe
Immunogène
Recombinant human NUP98 protein (a.a. 1-466, GST tag).
Hôte
Rat
Clonalité
Monoclonal
Clone
2H10
Isotype
IgG2c
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
96 kDa nucleoporin, 98 kDa nucleoporin, ADAR2, ADIR2, GLFG-repeat containing nucleoporin, Nuclear pore complex protein Nup96, Nuclear pore complex protein Nup98 Nup96, Nucleoporin 98kD, nucleoporin 98kDa, Nucleoporin Nup96, Nucleoporin Nup98, NUP196, NUP96, Nup98-Nup96, NUP98_HUMAN
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Detection of Nup98 protein by Western blotting using this antibody, 2H10. The sample is HeLa nuclear membrane fraction. The IgG was diluted 2,000 fold before use.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-NUP98 Antibody [2H10] (70-310)
Anti-NUP98 Antibody
Immunofluorescent staining of rat neuron with antibody 2H10. The dots are Nup98.Cliquez pour ouvrir le point de vue élargi
Figure 2: Anti-NUP98 Antibody [2H10] (70-310)
Anti-NUP98 Antibody
Detection of Np98 protein in whole cell extract of HeLa cells with 2H10 antibody.Cliquez pour ouvrir le point de vue élargi
Figure 3: Anti-NUP98 Antibody [2H10] (70-310)
Anti-NUP98 Antibody
Immunofluorescene staining of Nup98 in HeLa cells with 2H10 antibodies. Black-and-white images were obtained with mAb and DAPI. Color images represent merged images of mAb (green) with DAPI (magenta). HeLa cells were cultured in a glass-bottom dish for 2 days before fixation. The cells were fixed with cold methanol (-30?C) for 30 min. After washing with PBS, all fixed samples were blocked with 1% BSA for 2 hr at room temperature. Anti-Nup98 rat mAb 2H10 was diluted to 0.5 µg/ml in PBS. Blocked samples were treated with these primary antibody solutions overnight at 4?C. The secondary antibodies were 4 µg/ml of Alexa488-labelled anti-rat IgG. DNA was stained with 4',6-diamidino-2-phenylindole (DAPI). Samples were washed three times with PBS between treatments.Cliquez pour ouvrir le point de vue élargi
Figure 4: Anti-NUP98 Antibody [2H10] (70-310)
Anti-NUP98 Antibody

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