Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
214 kDa nucleoporin, CAIN, CAN, CAN protein putative oncogene, D9S46E, N214, NU214_HUMAN, Nuclear pore complex protein Nup214, nucleoporin 214kDa, Nucleoporin Nup214, p250, Protein CAN
Figure 1: Western Blot - Anti-NUP214 Antibody (A12242)
Western blot analysis of extracts of various cell lines, using Anti-NUP214 Antibody (A12242) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of L929 cells using Anti-NUP214 Antibody (A12242) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
