Anti-NuMA Antibody [ARC1000] (A308949)

Name: Anti-NuMA Antibody [ARC1000]
Brand: Antibodies.com
SKU: A308949
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1000] antibody to NuMA for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

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Centrosome Marker

Specifications

Name

Anti-NuMA Antibody [ARC1000]

Description

Rabbit monoclonal [ARC1000] antibody to NuMA.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2000-2100 of human NUMA11 (Q14980).

Sequence

TPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1000

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

NMP-22, NMP22, Nuclear matrix protein-22, Nuclear mitotic apparatus protein, Nuclear mitotic apparatus protein 1, NuMA protein, NUMA1, SP-H antigen

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-NuMA Antibody [ARC1000] (A308949)

Western blot analysis of extracts of various cell lines, using Anti-NuMA Antibody [ARC1000] (A308949) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-NuMA Antibody [ARC1000] (A308949)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-NuMA Antibody [ARC1000] (A308949) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NuMA Antibody [ARC1000] (A308949)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-NuMA Antibody [ARC1000] (A308949) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NuMA Antibody [ARC1000] (A308949)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NuMA Antibody [ARC1000] (A308949) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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