Mouse monoclonal [NCL/902] antibody to Nucleolin (CF® 488).
Spécificité
This antibody recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Applications
Flow Cytometry, IF
Recommander des dilutions
Flow Cytometry: 5-10 µl / million cells, IF: 1:50-1:100
Reactivité
Human
Réactivité croisée
This antibody does not cross react with Mouse, Rat, or Bovine.
Immunogène
Recombinant human NCL protein.
Hôte
Mouse
Clonalité
Monoclonal
Clone
NCL/902
Isotype
IgG1
Chaînes légères
kappa
Conjuguer
CF® 488
Excitation: 490nm, Emission: 515nm
Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Masse moléculaire
76 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Stockage
Store at 4-8°C. Antibody is stable for 24 months.
Synonymes
C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.