Recombinant Anti-Nucleolin Anticorps [NCL/8695R] (A315415)

Anti-Nucleolin Antibody [NCL/8695R]

Recombinant rabbit monoclonal [NCL/8695R] antibody to Nucleolin.

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

WB, IHC-P

All human cells. Human testis, ovary or Hodgkin s lymphoma.

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Recombinant full-length human NCL protein.

Rabbit

Monoclonal

NCL/8695R

kappa

Unconjugated

Protein A/G chromatography.

200 µg/ml

76 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23