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Anti-Nucleolin Anticorps (A15046)

Western Blot - Anti-Nucleolin Antibody (A15046) - Antibodies.com Immunohistochemistry - Anti-Nucleolin Antibody (A15046) - Antibodies.com
$335
Product Datasheet

Anticorps polyclonal de lapin contre Nucleolin pour WB et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Nucleolin Antibody
Description du produit
Rabbit polyclonal antibody to Nucleolin.
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivité
Human, Mouse
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 300-466 of human NCL (NP_005372.2).
Séquence
GTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGE
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
110 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Nucleolin Antibody (A15046) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Nucleolin Antibody (A15046)
Western blot analysis of extracts of various cell lines, using Anti-Nucleolin Antibody (A15046) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Nucleolin Antibody (A15046) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Nucleolin Antibody (A15046)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Nucleolin Antibody (A15046) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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