Anti-Nuclear Pore Complex Protéines Anticorps [39C7] (A85460)

Human HeLa cells were stained with Anti-Nuclear Pore Complex Antibody (green), which binds to a nuclear pore complex antigen, and Anti-Vimentin Antibody (A85421 | red). Note the close apposition of cytoplasmic vimentin intermediate filaments with the nuclear membrane, revealed by the Anti-Nuclear Pore Complex Antibody binding to the nuclear pores.

Immunofluorescent analysis of HeLa cells stained with Anti-Nuclear Pore Complex Antibody [39C7] (A85460), at a dilution of 1:100, in red, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Anti-Nuclear Pore Complex Antibody [39C7] (A85460) reveals strong granular staining of the nuclei corresponding to the NPC, while the Anti-Vimentin Antibody (A85421) specifically labels intermediate filaments in these cells.

Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions using Anti-Nuclear Pore Complex Antibody [39C7] (A85460), at a dilution of 1:100, in green. The lanes contain samples of: [1] Protein standards, in red, [2] HEK293 cytosol, [3] HEK293 nuclear, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear, [6] HeLa cytosol, and [7] HeLa nuclear fraction lysate. The band at about 68 kDa represents a currently unidentified NPC protein which is detected predominantly in the nuclear enriched fractions of all cell lines.