Figure 1: Western Blot - Anti-NPFF Antibody (A10045)
Western blot analysis of extracts of Rat plasma, using Anti-NPFF Antibody (A7020) at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.