Anti-NPAS4 Antibody [S408-79] (A305032)

Western blot analysis of rat brain showing detection of ~90 kDa NPAS4 protein using Anti-NPAS4 Antibody [S408-79] (A305032) at 1:1,000 for 16 hours at 4°C. Lane 1: MW Ladder. Lane 2: rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NPAS4 Antibody [S408-79] (A305032), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.