Anti-NLRP3 Anticorps (A82453)

Name: Anti-NLRP3 Antibody
Brand: Antibodies.com
SKU: A82453
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab4207). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre NLRP3 pour ELISA, IF, IHC et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-NLRP3 Antibody

Description du produit

Goat polyclonal antibody to NLRP3.

Applications

ELISA, IF, IHC, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, IHC: 4µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the C terminal region of human NLRP3.

Séquence

C-EKPELTVVFEPSW

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

AGTAVPRL, AII/AVP, Angiotensin/vasopressin receptor AII/AVP like, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, Caterpiller protein 1.1, CIAS 1, CIAS1, CLR1.1, Cold autoinflammatory syndrome 1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, Familial cold autoinflammatory syndrome, FCAS, FCU, LRR and PYD domains-containing protein 3, Muckle-Wells syndrome, MWS, NACHT, NACHT LRR and PYD containing protein 3, NALP 3, NALP3, NALP3_HUMAN, NLR family pyrin domain containing 3, PYPAF 1, PYPAF1, PYRIN containing APAF1 like protein 1, PYRIN-containing APAF1-like protein 1

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-NLRP3 Antibody (A82453)

NLRP3 expression in U937 cells analyzed by immunofluorescence. Cells immobilized on Shifix™ coverslip and permeabilized with 0.15% Triton. Staining was performed with Anti-NLRP3 Antibody (A82453) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane were stained and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-NLRP3 Antibody (A82453)

NLRP3 expression in U937 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NLRP3 Antibody (A82453) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-NLRP3 Antibody (A82453)

NLRP3 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-NLRP3 Antibody (A82453) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 4: Immunohistochemistry - Anti-NLRP3 Antibody (A82453)

Negative control for NLRP3 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.