Anti-NKCC1 Anticorps (A84750)

Name: Anti-NKCC1 Antibody
Brand: Antibodies.com
SKU: A84750
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab99558). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre NKCC1 pour ELISA, IHC, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-NKCC1 Antibody

Description du produit

Goat polyclonal antibody to NKCC1.

Applications

ELISA, IHC, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:64,000, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human NKCC1.

Séquence

C-NALQHSIRLSGVEDH

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Basolateral Na-K-Cl symporter, BSC, BSC2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, mBSC2, MGC104233, PPP1R141, Protein phosphatase 1, regulatory subunit 141, S12A2_HUMAN, Slc12a2, sodium-potassium-chloride cotransporter 1, Solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters) member 2, Solute carrier family 12 member 2, sy-ns

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Immunofluorescence - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle and membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NKCC1 Antibody (A84750) at 3.75µg/ml and revealed with alkaline phosphatase (AP).