Anti-Nitrotyrosine Anticorps [6B2-3G2] (A279468)

Anti-Nitrotyrosine Antibody [6B2-3G2]

Mouse monoclonal [6B2-3G2] antibody to Nitrotyrosine.

This antibody recognises both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro haemoglobin. The specificity as determined by competition ELISA is: Nitrotyrosine: 95 % of inhibition, Tyrosine: 0 % of inhibition, Nitrotryptophan: 4 % of inhibition, Aminotyrosine: 0 % of inhibition, Phosphotyrosine: 2 % of inhibition, Chlorotyrosine: 3 % of inhibition.

WB, ELISA, IP

ELISA: 2 - 100ng/ml, IP: 1 µg/ml, WB: 0.1 µg/ml

Species Independent

Protein-bound Nitro-Tyrosine.

Mouse

Monoclonal

6B2-3G2

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, inflammatory bowel disease and cystic fibrosis.