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Anti-NFkB p105/p50 Antibody (A80434)

Western Blot - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.com Immunohistochemistry - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.com Immunofluorescence - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.com Western Blot - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to NFkB p105/p50 for WB, IHC, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-NFkB p105/p50 Antibody
Description
Rabbit polyclonal antibody to NFkB p105/p50.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:20-1:100, IP: 1:20-1:50
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 41-365 of NFKB1 (NP_001158884.1).
Sequence
GPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.comEnlarge Image

Western Blot - Anti-NFkB p105/p50 Antibody (A80434)

Western blot analysis of extracts of Raji cells, using Anti-NFkB p105/p50 Antibody (A80434) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-NFkB p105/p50 Antibody (A80434)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-NFkB p105/p50 Antibody (A80434) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-NFkB p105/p50 Antibody (A80434)

Immunofluorescence analysis of MCF7 cells using Anti-NFkB p105/p50 Antibody (A80434).
Western Blot - Anti-NFkB p105/p50 Antibody (A80434) - Antibodies.comEnlarge Image

Western Blot - Anti-NFkB p105/p50 Antibody (A80434)

Immunoprecipitation analysis of 150µg extracts of MCF-7 cells using 3µg of Anti-NFkB p105/p50 Antibody (A80434). This Western blot was performed on the immunoprecipitate using Anti-NFkB p105/p50 Antibody (A80434) at a dilution of 1:500.
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