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NFIL3 Cell Based Kit Elisa (A103169)

Nom du produit
NFIL3 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This NFIL3 Cell Based ELISA Kit allows for the detection of NFIL3 and the effects that certain stimulation conditions have on NFIL3 expression in different cell lines. Qualitative determination of NFIL3 concentration is achieved by an indirect ELISA format. In essence, the NFIL3 is captured by Anti-NFIL3 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this NFIL3 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
E4 promoter binding protein 4, E4 promoter-binding protein 4, E4BP 4, E4BP4, E4BP4 protein, IL3BP 1, IL3BP1, Interleukin 3 binding protein 1, Interleukin 3 promoter transcriptional activator, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, NFIL 3, NFIL 3A, NFIL3A, NFIL3_HUMAN, Nuclear factor interleukin 3 regulated, Nuclear factor interleukin-3-regulated protein, Transcriptional activator NF IL3A, Transcriptional activator NF-IL3A
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - NFIL3 Cell Based ELISA Kit (CB6038) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - NFIL3 Cell Based ELISA Kit (CB6038)
Western blot analysis of extracts from 293/K562 cells, using Anti-NFIL3 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - NFIL3 Cell Based ELISA Kit (CB6038) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: NFIL3 Cell Based ELISA Kit (CB6038)
A diagram showing the protocol for NFIL3 Cell Based ELISA Kit.
Protocol Summary - NFIL3 Cell Based ELISA Kit (CB6038) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: NFIL3 Cell Based ELISA Kit (CB6038)
A summary of the protocol for NFIL3 Cell Based ELISA Kit.
Western Blot - Control Antibody - NFIL3 Cell Based ELISA Kit (CB6038) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - NFIL3 Cell Based ELISA Kit (CB6038)
The mouse monoclonal antibody to GAPDH is used as a positive control inNFIL3 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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