Anti-NF-kB p65 (phospho Ser536) Anticorps (A16453)

Name: Anti-NF-kB p65 (phospho Ser536) Antibody
Brand: Antibodies.com
SKU: A16453
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre NF-kB p65 (phospho Ser536) pour WB.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-NF-kB p65 (phospho Ser536) Antibody

Description du produit

Rabbit polyclonal antibody to NF-kB p65 (phospho Ser536).

Applications

Recommander des dilutions

WB: 1:2,000-1:10,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S536 of human RelA (NP_068810.3).

Séquence

FSSIA

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

65 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-NF-kB p65 (phospho Ser536) Antibody (A16453)

Western blot analysis of HeLa, using Anti-NF-kB p65 (phospho Ser536) Antibody (A16453) at 1:10,000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-NF-kB p65 (phospho Ser536) Antibody (A16453)

Western blot analysis of NIH/3T3, using Anti-NF-kB p65 (phospho Ser536) Antibody (A16453) at 1:10,000 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-NF-kB p65 (phospho Ser536) Antibody (A16453)

Western blot analysis of C6, using Anti-NF-kB p65 (phospho Ser536) Antibody (A16453) at 1:10,000 dilution. C6 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.