Rabbit polyclonal antibody to Neutrophil Cytosol Factor 1 (Phospho-Ser304)
Specificity
The antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 304(R-S-S(p)-I-R) derived from Human Neutrophil Cytosol Factor 1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody #11703. The lane on the left is treated with antigen-specific peptide.
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