Figure 1: Western Blot - Anti-Neuroligin 2 Antibody (A84310)
Neuroligin 2 expression in Human Pancrease lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Neuroligin 2 Antibody (A84310) at 2µg/ml and detected by chemiluminescence.
Neuroligin 2 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Neuroligin 2 Antibody (A84310) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Neuroligin 2 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Neuroligin 2 Antibody (A84310) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
Neuroligin 2 expression in Human Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Neuroligin 2 Antibody (A84310) at 5µg/ml and revealed with alkaline phosphatase (AP). Note this data is from a previous batch and is not on sale.
Produits alternatifs à Anti-Neuroligin 2 Anticorps (A84310)
