Anti-NeuroG3 Anticorps [PCRP-NEUROG3-1E10] (A278318)

Name: Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free
Brand: Antibodies.com
SKU: A278318
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [PCRP-NEUROG3-1E10] de souris contre NeuroG3 pour ELISA, IP et Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Monospécifique

Spécifications

Nom du produit

Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free

Description du produit

Mouse monoclonal [PCRP-NEUROG3-1E10] antibody to NeuroG3.

Spécificité

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Applications

ELISA, IP, Flow Cytometry

Recommander des dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells

Reactivité

Human

Immunogène

Recombinant full-length human NeuroG3 protein.

Hôte

Mouse

Clonalité

Monoclonal

Clone

PCRP-NEUROG3-1E10

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Masse moléculaire

23.54 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] (A277730).

Synonymes

ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, Neurogenin-3, Neurogenin3, NGN-3, ngn3, NGN3_HUMAN, OTTHUMP00000019730, Protein atonal homolog 5

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Flow Cytometry - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 2: Immunofluorescence - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

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Figure 3: SDS-PAGE - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

SDS-PAGE analysis of Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 4: Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.