Anti-NeuN Antibody (A92020)

Name: Anti-NeuN Antibody
Brand: Antibodies.com
SKU: A92020

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This product is discontinued

Anti-NeuN Antibody (A92020) has been discontinued and is no longer available.

View all NeuN Antibodies.

Specifications

Name

Anti-NeuN Antibody

Description

Rabbit polyclonal antibody to NeuN.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076045.1).

Sequence

MEKKKMVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Fox-1 homolog C, NeuN antigen, Neuronal nuclei antigen, RBFOX3, RNA binding protein fox-1 homolog 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-NeuN Antibody (A92020)

Western blot analysis of extracts of U-251MG cells, using Anti-NeuN Antibody (A92020) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-NeuN Antibody (A92020)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-NeuN Antibody (A92020) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NeuN Antibody (A92020)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-NeuN Antibody (A92020) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NeuN Antibody (A92020)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NeuN Antibody (A92020) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.