Anti-NCKAP1 Anticorps (A326266)

Name: Anti-NCKAP1 Antibody
Brand: Antibodies.com
SKU: A326266
Price: 448.0 USD
Availability: InStock

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$448

Anticorps polyclonal de chèvre contre NCKAP1 pour ELISA, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-NCKAP1 Antibody

Description du produit

Goat polyclonal antibody to NCKAP1.

Applications

ELISA, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human NCKAP1.

Séquence

C-TPREYLTSHLEIR

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

100 kDa

MW prédit

129kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

FLJ11291, KIAA0587, Membrane associated protein HEM2, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP1, NAP125, NCK associated protein, NCK associated protein 1, Nck-associated protein 1, NCKP1_HUMAN, OTTHUMP00000163460, OTTHUMP00000163461, p125Nap1

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Immunofluorescence - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.