Western Blot - Anti-Myosin Phosphatase Antibody (A80617)
Western blot analysis of extracts of various cell lines, using Anti-Myosin Phosphatase Antibody (A80617) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using Anti-Myosin Phosphatase Antibody (A80617) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Myosin Phosphatase Antibody (A80617) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-Myosin Phosphatase Antibody (A80617) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-Myosin Phosphatase Antibody (A80617) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Myosin Phosphatase Antibody (A80617)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Myosin Phosphatase Antibody (A80617). This Western blot was performed on the immunoprecipitate using Anti-Myosin Phosphatase Antibody (A80617) at a dilution of 1:1000.
