Anti-Myosin Antibody (A88758)

Name: Anti-Myosin Antibody
Brand: Antibodies.com
SKU: A88758
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Myosin for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Myosin Antibody

Description

Rabbit polyclonal antibody to Myosin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1450-1550 of human MYH4 (NP_060003.2).

Sequence

QRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cardiac myosin light chain 1, CMLC1, MLC-lV/sb, MLC1SB, MYL3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, Myosin light chain 3, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, VLCl

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Myosin Antibody (A88758)

Western blot analysis of Mouse skeletal muscle, using Anti-Myosin Antibody (A88758) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-Myosin Antibody (A88758)

Western blot analysis of various lysates, using Anti-Myosin Antibody (A88758) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Immunohistochemistry - Anti-Myosin Antibody (A88758)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Myosin Antibody (A88758) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Myosin Antibody (A88758)

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using Anti-Myosin Antibody (A88758) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.